Difference between revisions of "Part:BBa I716000:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
 +
<pre>
 
Bbrick ORF wbbL
 
Bbrick ORF wbbL
 
[pcr] wbbL-F/R on pJ23006-Bca9106        (826bp, BglII/XhoI)
 
[pcr] wbbL-F/R on pJ23006-Bca9106        (826bp, BglII/XhoI)
Line 15: Line 16:
 
AY001.wbbL-F    ccaagAGATCTatggtatatataataatcg
 
AY001.wbbL-F    ccaagAGATCTatggtatatataataatcg
 
AY002.wbbL-R    gctagCTCGAGttaGGATCCttacgggtgaaaaactgatg
 
AY002.wbbL-R    gctagCTCGAGttaGGATCCttacgggtgaaaaactgatg
−
 
+
</pre>
−
 
+
  
 
===Source===
 
===Source===

Revision as of 18:33, 6 June 2007


wbbL


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Bbrick ORF wbbL
[pcr] wbbL-F/R on pJ23006-Bca9106        (826bp, BglII/XhoI)
[sub] into pBca9145-Bca1144#5            (BglII/XhoI, 2063+910, L)
[prod] pBca9145-wbbL (I716000)

---
AY001.wbbL-F     ccaagAGATCTatggtatatataataatcg
AY002.wbbL-R     gctagCTCGAGttaGGATCCttacgggtgaaaaactgatg

Source

pJ23006-Bca9106

References