Difference between revisions of "Part:BBa I716251:Design"
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No Oligos Needed | No Oligos Needed | ||
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===Source=== | ===Source=== | ||
Latest revision as of 15:50, 6 June 2007
Composite part consisting of Ptet + RFP
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1
Illegal PstI site found at 785 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal PstI site found at 785 - 21INCOMPATIBLE WITH RFC[21]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 770 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1
Illegal PstI site found at 785 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1
Illegal PstI site found at 785
Illegal AgeI site found at 649
Illegal AgeI site found at 761 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1872
Design Notes
Biobrickificreation of composite Ptet + RFP (product = I716251)
Digest pBca9145-Bca1145 (BglII/BsaI, 1783+970, L) Sub into pBca9145-Bca9205 (BamHI/BsaI/AlwNI, 1049+553+449, L) Product is I716251
No Oligos Needed
This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites.
Source
The source of these parts are:
pBca9145-Bca1145
pBca9145-Bca9205