Difference between revisions of "Part:BBa K2036018"

Revision as of 07:29, 29 September 2016

pRE-RBS-Cro-RBS-CII-TT-ptrp2

This is a part of Prokaryote version of Signal Filter characterization circuit:pRE-RBS-Cro-RBS-CII-TT-ptrp-RBS-CI-TT-pR-RBS-CIII-RBS-RFP-LAAssrAtag-TT-pRM-RBS-GFP-LVAssrAtag (BBa_K2036027).

Fig1:Prokaryote version of Signal Filter characterization circuit

Because the circuit is too complex, we divided it into three parts and each is assembled by In-Fusion method (homologous recombination). The whole circuit is built by 3A assembly and then we transfer the backbone from pSB1C3 to PET-Duet-1.

Fig2:Prokaryote version of Signal Filter characterization plasmid

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 This is a part of Prokaryote version of Signal Filter characterization circuit:pRE-RBS-Cro-RBS-CII-TT-ptrp-RBS-CI-TT-pR-RBS-CIII-RBS-RFP-LAAssrAtag-TT-pRM-RBS-GFP-LVAssrAtag ([https://parts.igem.org/Part:BBa_K2036027 BBa_K2036027]).
 [[File:T--HUST-China--Experiments-Fig14-1.png|thumb|500px|center|Fig1:Prokaryote version of Signal Filter characterization circuit]]
-<br>Because the circuit is too complex we divided it into three parts and each is assembled by In-Fusion method (homologous recombination). The whole circuit built by 3A assembly and then we transfer the backbone from pSB1C3 to PET-Duet-1.
+<br>Because the circuit is too complex, we divided it into three parts and each is assembled by In-Fusion method (homologous recombination). The whole circuit is built by 3A assembly and then we transfer the backbone from pSB1C3 to PET-Duet-1.
 [[File:T--HUST-China--Experiments-Fig13.png|thumb|350px|center|Fig2:Prokaryote version of Signal Filter characterization plasmid]]
 <!-- Add more about the biology of this part here