Difference between revisions of "Part:BBa K2036017"

 
Line 3: Line 3:
 
<partinfo>BBa_K2036017 short</partinfo>
 
<partinfo>BBa_K2036017 short</partinfo>
  
CI-TT-pR-RBS-GFP-LVAtag
+
pR is a lytic promoter with two binding sites OR1* and OR2 * which can be recognized by CI. The circuit is built to characterize CI and pR interaction together with control group: pR-GFp-LVAssrAtag ([https://parts.igem.org/Part:BBa_K2036016 BBa_K2036016]).
 
+
[[File:T--HUST-China--Experiments-Fig11.png|thumb|500px|center|Fig1:CI and pR interaction characterization circuits]]
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 05:43, 29 September 2016


CI-TT-pR-RBS-GFP-LVAssrAtag

pR is a lytic promoter with two binding sites OR1* and OR2 * which can be recognized by CI. The circuit is built to characterize CI and pR interaction together with control group: pR-GFp-LVAssrAtag (BBa_K2036016).

Fig1:CI and pR interaction characterization circuits

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1604