Difference between revisions of "Part:BBa K2036015"

Revision as of 05:13, 29 September 2016

RBS-CII-RBS-CII-RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag
CII acts as a necessary and sufficient cis-acting target for rapid proteolysis by initiating pRE. The circuit is built to test CII and pRE interaction together with RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag ([ https://parts.igem.org/Part:BBa_K2036013 BBa_K2036013 ]) and pRE-RBS-GFP-LVAssrAtag ( [ https://parts.igem.org/Part:BBa_K2036011 BBa_K2036011 ]) . Considering Ftsh protease degrades it raidly in the host, RBS-CII-RBS-CII tandom expression circuit is constructed.

Fig1:CII and pRE interaction characterization circuits

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1770

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 <partinfo>BBa_K2036015 short</partinfo>
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-CII acts as a necessary and sufficient cis-acting target for rapid proteolysis by initiating pRE. The circuit is built to test CII and pRE interaction together with RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag  ([https://parts.igem.org/Part:BBa_K2036013 BBa_K2036013]) and pRE-RBS-GFP-LVAssrAtag ( [https://parts.igem.org/Part:BBa_K2036011 BBa_K2036011]) . Considering Ftsh protease degrades it raidly in the host, RBS-CII-RBS-CII tandom expression circuit is constructed.
+CII acts as a necessary and sufficient cis-acting target for rapid proteolysis by initiating pRE. The circuit is built to test CII and pRE interaction together with RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag  ([ https://parts.igem.org/Part:BBa_K2036013 BBa_K2036013 ]) and pRE-RBS-GFP-LVAssrAtag ( [ https://parts.igem.org/Part:BBa_K2036011 BBa_K2036011 ]) . Considering Ftsh protease degrades it raidly in the host, RBS-CII-RBS-CII tandom expression circuit is constructed.
 [[File:T--HUST-China--Experiments-Fig10.png |thumb|500px|center|Fig1:CII and pRE interaction characterization circuits]]