Difference between revisions of "Part:BBa K2055007:Design"

 
(→‎Source)
Line 12: Line 12:
  
 
===Source===
 
===Source===
−
 
+
GenBank: AII71792.1
−
The RBS used is the BBa_B0034, the terminator is the BBA_B0012, the LacI reculated Promoter is the BBa_R0011.
+
−
The sequence for the FUR Box was obtained from the results of the paper of Quatrini et al. (Citation in our wiki), choosing the Fur box with the best score.
+
−
The sequence for the FUR protein was obtained from the Acidithiobacillus ferrooxidans genome.  
+
  
 
===References===
 
===References===

Revision as of 21:00, 28 September 2016


Gold/copper inducible regulator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 93
    Illegal AgeI site found at 1086
    Illegal AgeI site found at 1198
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It does not have a promoter.


Source

GenBank: AII71792.1

References