Difference between revisions of "Part:BBa K2036014"

Revision as of 11:22, 28 September 2016

RBS-CIII-RBS-CIII-RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag

CIII acts as an inhibitor of the ubiquitous Escherichia coli metalloprotease HflB (FtsH). This inhibition results in the stabilization of transcription factor lambda CII, thereby helping the phage to lysogenize the host bacterium.
This circuit is built to characterize CIII's function comparing to control group: RBS-CII-RBS-CII-RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag (BBa_K2036015)

Fig1: CIII characterization circuit-test group

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1550

@@ Line 5: / Line 5: @@
 CIII acts as an inhibitor of the ubiquitous Escherichia coli metalloprotease HflB (FtsH). This inhibition results in the stabilization of transcription factor lambda CII, thereby helping the phage to lysogenize the host bacterium.
 <br>This circuit is built to characterize CIII's function comparing to control group: RBS-CII-RBS-CII-RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag ([https://parts.igem.org/Part:BBa_K2036015 BBa_K2036015])
-[[File:T--HUST-China--Experiments-Fig6.png|thumb|500px|center]]
+[[File:T--HUST-China--Experiments-Fig6.png|thumb|500px|center|Fig1: CIII characterization circuit-test group]]
 <!-- Add more about the biology of this part here
 ===Usage and Biology===