Difference between revisions of "Part:BBa K2036013"

 
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<partinfo>BBa_K2036013 short</partinfo>
 
<partinfo>BBa_K2036013 short</partinfo>
  
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RBS-CII-TT-pRE-RBS-GFP-LVAtag, it is a GFP regulationary circuits. When CII is expressed it can further activate GFP and forming a Switching Lag-times.
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It is a GFP regulationary circuits. When CII is expressed it can further activate GFP and forming a Switching Lag-times.
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HUST-China 2016 build this part to characterize CII and pRE interaction with a control group:pRE-GFP-LVAssrAtag ( [https://parts.igem.org/Part:BBa_K2036011 BBa_K2036011]). And we also construct a tandem expression of CII ([https://parts.igem.org/Part:BBa_K2036015 BBa_K2036015])to figure out if the flouresence level will come up with CII.
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[[File:T--HUST-China--Experiments-Fig10.png|thumb|500px|center|Fig1:CII&pRE interaction characterization circuits]]
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 08:05, 28 September 2016


RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag

It is a GFP regulationary circuits. When CII is expressed it can further activate GFP and forming a Switching Lag-times. HUST-China 2016 build this part to characterize CII and pRE interaction with a control group:pRE-GFP-LVAssrAtag ( BBa_K2036011). And we also construct a tandem expression of CII (BBa_K2036015)to figure out if the flouresence level will come up with CII.

Fig1:CII&pRE interaction characterization circuits

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1168