Difference between revisions of "Part:BBa K2036011"

 
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__NOTOC__
 
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<partinfo>BBa_K2036011 short</partinfo>
 
<partinfo>BBa_K2036011 short</partinfo>
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It is a GFP generator,and the production of GFP will be activated by a certain level of CII or CII with help of CIII in E.coli.
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pRE-GFP-LVAtag, it is a GFP generator,and the production of GFP will be activated by CII and CIII.
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HUST-China 2016 built this circuit to characterize CII and pRE interaction with test group:RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag ([https://parts.igem.org/Part:BBa_K2036013 BBa_K2036013])and RBS-CII-RBS-CII-RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag ([https://parts.igem.org/Part:BBa_K2036015 BBa_K2036015])
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[[File: T--HUST-China--Experiments-Fig10.png|thumb|400px|center|Fig1:CII&pRE interaction characterization circuit]]
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 07:52, 28 September 2016


pRE-GFP-LVAssrAtag It is a GFP generator,and the production of GFP will be activated by a certain level of CII or CII with help of CIII in E.coli. HUST-China 2016 built this circuit to characterize CII and pRE interaction with test group:RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag (BBa_K2036013)and RBS-CII-RBS-CII-RBS-CII-TT-pRE-RBS-GFP-LVAssrAtag (BBa_K2036015)

Fig1:CII&pRE interaction characterization circuit

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 719