Difference between revisions of "Part:BBa K2036010"

Revision as of 07:43, 28 September 2016

Cro-TT-pRM-RBS-GFP-LVAssrAtag

It is a negtive control of GFP expression. When inserted into a expression plasmid behind an inducible promoter,it will form a NO gate. Take PETDuet-1 as an example,after constructing PETDurt-1-Cro-TT-pRM-RBS-GFP-LVAssrAtag,IPTG can trigger T7 promoter in the plasmid, and cro can bind to a certain site within pRM, so that GFP will not be produced. And we get a NO gate of GFP generator. HUST-Chian 2016 Build this circuit to test Cro and pRM interaction intensity with contol group:pRM-GFP-LVAssrAtag (BBa_K2036009)

Fig1:Cro&pRM interaction characterization circuit

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1036

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 <partinfo>BBa_K2036010 short</partinfo>
-Cro-TT-pRM-RBS-GFP-LVAtag, it is a negtive control of GFP expression. When inserted into a expression plasmid behind an inducible promoter,it will form a NO gate. Take PETDuet-1 as an example, IPTG can trigger T7 promoter in the plasmid, and cro can bind to a certain site within pRM, so that GFP will not be produced. And we get a NO gate of GFP generator.
+It is a negtive control of GFP expression. When inserted into a expression plasmid behind an inducible promoter,it will form a NO gate. Take PETDuet-1 as an example,after constructing PETDurt-1-Cro-TT-pRM-RBS-GFP-LVAssrAtag,IPTG can trigger T7 promoter in the plasmid, and cro can bind to a certain site within pRM, so that GFP will not be produced. And we get a NO gate of GFP generator.
+HUST-Chian 2016 Build this circuit to test Cro and pRM interaction intensity with contol group:pRM-GFP-LVAssrAtag ([https://parts.igem.org/Part:BBa_K2036009 BBa_K2036009])
 [[File:T--HUST-China--Experiments-Fig12.png|thumb|400px|center|Fig1:Cro&pRM interaction characterization circuit]]