Difference between revisions of "Part:BBa K2036010"

 
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<partinfo>BBa_K2036010 short</partinfo>
 
<partinfo>BBa_K2036010 short</partinfo>
  
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Cro-TT-pRM-RBS-GFP-LVAtag, it is a negtive control of GFP expression. When inserted into a expression plasmid behind an inducible promoter,it will form a NO gate: GFP will not be produced when there is an induction.
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Cro-TT-pRM-RBS-GFP-LVAtag, it is a negtive control of GFP expression. When inserted into a expression plasmid behind an inducible promoter,it will form a NO gate. Take PETDuet-1 as an example, IPTG can trigger T7 promoter in the plasmid, and cro can bind to a certain site within pRM, so that GFP will not be produced. And we get a NO gate of GFP generator.
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[[File:T--HUST-China--Experiments-Fig12.png|thumb|400px|center|Fig1:Cro&pRM interaction characterization circuit]]
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 07:36, 28 September 2016


Cro-TT-pRM-RBS-GFP-LVAssrAtag

Cro-TT-pRM-RBS-GFP-LVAtag, it is a negtive control of GFP expression. When inserted into a expression plasmid behind an inducible promoter,it will form a NO gate. Take PETDuet-1 as an example, IPTG can trigger T7 promoter in the plasmid, and cro can bind to a certain site within pRM, so that GFP will not be produced. And we get a NO gate of GFP generator.

Fig1:Cro&pRM interaction characterization circuit

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1036