Difference between revisions of "Part:BBa K2036009"

Revision as of 07:35, 28 September 2016

pRM-GFP-LVAssrAtag

pRM-GFP-LVAtag, the circuit can function as a control group of Cro and pRM interaction characterization with test group: Cro-TT-pRM-RBS-GFP-LVAssrAtag (BBa_K20360010).

Fig1:Cro&pRM interaction characterization circuit

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 699

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 <partinfo>BBa_K2036009 short</partinfo>
-pRM-GFP-LVAtag, the circuit can function as control group of Cro and pRM interaction characterization with test group: Cro-TT-pRM-RBS-GFP-LVAssrAtag ([https://parts.igem.org/Part:BBa_K20360010 BBa_K20360010]).
+pRM-GFP-LVAtag, the circuit can function as a control group of Cro and pRM interaction characterization with test group: Cro-TT-pRM-RBS-GFP-LVAssrAtag ([https://parts.igem.org/Part:BBa_K20360010 BBa_K20360010]).
-<br>When inserted into expression plasmid and tramsformed into E.coli. Take PETDuet-1 as an example, IPTG can trigger T7 promoter in the plasmid, and  cro can bind to a certain site within pRM, so that GFP will not be produced. And we get a NO gate of GFP generator.
 [[File:T--HUST-China--Experiments-Fig12.png|thumb|400px|center|Fig1:Cro&pRM interaction characterization circuit]]