Difference between revisions of "Part:BBa K2036027:Design"
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===Design Notes=== | ===Design Notes=== | ||
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| + | Feedback control mechanism,protein-protein interactions,protein-promoter interactions. | ||
===Source=== | ===Source=== | ||
Latest revision as of 09:10, 25 September 2016
pRE-RBS-Cro-RBS-CII-TT-ptrp-RBS-CI-TT-pR-RBS-CIII-RBS-RFP-LAAssrAtag-TT-pRM-RBS-GFP-LVAssrAtag
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 717
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 717
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 717
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 717
Illegal AgeI site found at 2533
Illegal AgeI site found at 2645 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3561
Design Notes
Feedback control mechanism,protein-protein interactions,protein-promoter interactions.
Source
Assembly
References
[1]Schubert R A, Dodd I B, Egan J B, et al. Cro’s role in the CI–Cro bistable switch is critical for λ’s transition from lysogeny to lytic development[J]. Genes & development, 2007, 21(19): 2461-2472.
[2]Halder S, Datta A B, Parrack P. Probing the antiprotease activity of λCIII, an inhibitor of the Escherichia coli metalloprotease HflB (FtsH)[J]. Journal of bacteriology, 2007, 189(22): 8130-8138.
[3]Marr M T, Roberts J W, Brown S E, et al. Interactions among CII protein, RNA polymerase and the λ PRE promoter: contacts between RNA polymerase and the–35 region of PRE are identical in the presence and absence of CII protein[J]. Nucleic acids research, 2004, 32(3): 1083-1090.