Difference between revisions of "Part:BBa K1555000:Experience"

Revision as of 11:54, 23 September 2016

This sensor can be activated in low copper concentration and it's truely useful.

2h 0mg/L copper ion 2h 1mg/L copper ion

Applications of BBa_K1555000

User Reviews

UNIQa2c89d0cdf89091d-partinfo-00000000-QINU

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HSiTAIWAN

Detecting Copper in Chinese medicine

Objective

Measure the E. coli fluorescence intensity in different copper ion concentration with 3μg/ml Chinese medicine.

Methods

Copper ion solution with different copper ion concentration (0, 3, 9, 27ppm) is added into E. coli DH5α with Plasmids encoding GFP under copper promoter(part: BBa_K1555000).
Fluorescence was measured at:

Excitation peak: 470nm
Emission peak: 508nm

every 30 minutes.

Results

This part is still specific to copper in Chinese medicine, so it be apply in Chinese medicine examination.

Discussion

Since E. coli expresses stronger fluorescence in higher copper concentration, this is a relevant promoter to detect the existence and measure the amount of copper.

Review

This part works great! HSiTAIWAN iGEM team 2016 used this part to detect copper ions in Chinese Medicine. The results were as expected. The reporter performed well without being interfered by complex substances. This part performed in an expected pattern and is an useful reporter.

UNIQa2c89d0cdf89091d-partinfo-00000002-QINU

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 	<h4>Discussion</h4>
-	        &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Since E. coli expresses stronger fluorescence in higher copper concentration, this is a relevant promoter to detect the existence and measure the amount of arsenic.
+	        &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Since E. coli expresses stronger fluorescence in higher copper concentration, this is a relevant promoter to detect the existence and measure the amount of copper.
 	<h4>Review</h4>