Difference between revisions of "Part:BBa J100300:Experience"
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Promoter was ligated during Golden Gate Assembly into E. coli plasmid of strain JM109. Promoter was upstream of RFP (red fluorescent protein) gene. Experiment included positive control with strong known promoter, negative control without a functioning promoter, and two experimental groups with and without 2MC (2-methylcitrate). Data was measured in fluorescence to cell density ration. Data shows that PprpB is non-functioning regardless of presence of 2MC. | Promoter was ligated during Golden Gate Assembly into E. coli plasmid of strain JM109. Promoter was upstream of RFP (red fluorescent protein) gene. Experiment included positive control with strong known promoter, negative control without a functioning promoter, and two experimental groups with and without 2MC (2-methylcitrate). Data was measured in fluorescence to cell density ration. Data shows that PprpB is non-functioning regardless of presence of 2MC. | ||
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===Applications of BBa_J100300=== | ===Applications of BBa_J100300=== | ||
Revision as of 14:03, 22 September 2016
Promoter was ligated during Golden Gate Assembly into E. coli plasmid of strain JM109. Promoter was upstream of RFP (red fluorescent protein) gene. Experiment included positive control with strong known promoter, negative control without a functioning promoter, and two experimental groups with and without 2MC (2-methylcitrate). Data was measured in fluorescence to cell density ration. Data shows that PprpB is non-functioning regardless of presence of 2MC.
Applications of BBa_J100300
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