Difference between revisions of "Part:BBa K1994000"

 
 
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<partinfo>BBa_K1994000 short</partinfo>
 
<partinfo>BBa_K1994000 short</partinfo>
  
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Part containing only dCas9 enzyme with no terminator or promoter. Interacts with sgRNA to bind to specific DNA target.
+
Part containing only dCas9 enzyme with no terminator or promoter. For use in CRISPR/Cas9 systems. Interacts with sgRNA to bind to specific DNA target. Nuclease deficient version of Cas9 enzyme.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 08:59, 22 September 2016


dCas9 enzyme

Part containing only dCas9 enzyme with no terminator or promoter. For use in CRISPR/Cas9 systems. Interacts with sgRNA to bind to specific DNA target. Nuclease deficient version of Cas9 enzyme.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1096
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3375
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]