Difference between revisions of "Part:BBa K2033000:Design"
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===Design Notes=== | ===Design Notes=== | ||
| + | The original AubI part was sampled from soil and is thought to be from an unknown soil-borne bacteria. The bacteria was transformed into BL21 Competent E. coli cells to produce sufficient stock for future experiments. Of the 70 colonies that were produced, three samples were taken for verification. After mini-prepping, the gel verification affirmed the identity of the sample, as shown below: | ||
| + | <div style="text-align: center;">[[File:T--Arizona State--Gel4.jpg]]</div> | ||
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| + | <div style="text-align: center;">Lanes 3,4,5 correspond to AubI samples; Lane 1 contains the kb+ ladder./div> | ||
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===Source=== | ===Source=== | ||
This synthase was recovered from a metagenomic project on a soil sample. The specific source is unknown. | This synthase was recovered from a metagenomic project on a soil sample. The specific source is unknown. | ||
Revision as of 08:24, 19 September 2016
N-dodecanoyl-L-homoserine lactone (C(12)-HSL) Sender- AubI
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 535
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 510
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The original AubI part was sampled from soil and is thought to be from an unknown soil-borne bacteria. The bacteria was transformed into BL21 Competent E. coli cells to produce sufficient stock for future experiments. Of the 70 colonies that were produced, three samples were taken for verification. After mini-prepping, the gel verification affirmed the identity of the sample, as shown below:
Lanes 3,4,5 correspond to AubI samples; Lane 1 contains the kb+ ladder./div>
Source
This synthase was recovered from a metagenomic project on a soil sample. The specific source is unknown.