Difference between revisions of "Part:BBa K2036001:Design"
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===References=== | ===References=== | ||
| + | Halder S, Datta A B, Parrack P. Probing the antiprotease activity of λCIII, an inhibitor of the Escherichia coli metalloprotease HflB (FtsH)[J]. Journal of bacteriology, 2007, 189(22): 8130-8138. | ||
Latest revision as of 05:43, 19 September 2016
CIII
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
It can inhibit Ftsh to degrade CII,thus protecting CII to initiate pRE and express downstream gene. One can directly insert CIII into MCS of a expression plasmid which contains promoter and terminator. And considering that Ftsh normally exists in E.coli, sometimes we can try over express CIII to guarantee CII’s function.
Source
come from bacteriaphage lambda operon
References
Halder S, Datta A B, Parrack P. Probing the antiprotease activity of λCIII, an inhibitor of the Escherichia coli metalloprotease HflB (FtsH)[J]. Journal of bacteriology, 2007, 189(22): 8130-8138.