Difference between revisions of "Part:BBa J100301"
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| − | The promoter ompW is regulated by the inhibitor protein ArcA. In the presence of hydrogen peroxide, ArcA is activated and, therefore, ompW is inhibited. For our project, we will use a 56-base pair long sequence of DNA that we will place into a receiving plasmid using BsaI sticky ends. We will then use bacterial transformation to determine the functionality of our promoter sequence. | + | The promoter ompW is regulated by the inhibitor protein ArcA. In the presence of 4mM hydrogen peroxide, ArcA is activated and, therefore, ompW is inhibited. For our project, we will use a 56-base pair long sequence of DNA that we will place into a receiving plasmid using BsaI sticky ends. We will then use bacterial transformation to determine the functionality of our promoter sequence. |
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Revision as of 14:23, 15 September 2016
ompW Promoter
The promoter ompW is regulated by the inhibitor protein ArcA. In the presence of 4mM hydrogen peroxide, ArcA is activated and, therefore, ompW is inhibited. For our project, we will use a 56-base pair long sequence of DNA that we will place into a receiving plasmid using BsaI sticky ends. We will then use bacterial transformation to determine the functionality of our promoter sequence.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]