Difference between revisions of "Part:BBa K2012003:Design"

 
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
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The bc3 riboswitch is apart of the tandem riboswitch bc3-5. We analysed the sequence of bc3-5 and its scendary structure, found linkers between three complete riboswitches. Using PCR amplify the bc3 sequence which is similar with bc4 and bc5 with prefix and suffix, whereas the secondary structure of bc3 is different from other riboswitches'.
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The bc4 riboswitch is apart of the tandem riboswitch bc3-5. We analysed the sequence of bc3-5 and its scendary structure, found linkers between three complete riboswitches. Using PCR amplify the bc3 sequence which is similar with bc4 and bc5 with prefix and suffix, whereas the secondary structure of bc3 is different from other riboswitches'.
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===Source===
 
===Source===

Latest revision as of 06:14, 15 September 2016


c-di-GMP tandem riboswitche bc4


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The bc4 riboswitch is apart of the tandem riboswitch bc3-5. We analysed the sequence of bc3-5 and its scendary structure, found linkers between three complete riboswitches. Using PCR amplify the bc3 sequence which is similar with bc4 and bc5 with prefix and suffix, whereas the secondary structure of bc3 is different from other riboswitches'.

Source

In the CT-43 genome(GenBank accession: NC_017208.1), five type I c-di-GMP riboswitches named Bc1, Bc2, Bc3, Bc4 and Bc5 RNAwere annotated in the Rfam database (http://rfam.sanger.ac.uk/search)42. While Bc1 and Bc2 are widely distributed in the B. cereus group4, Bc3, Bc4, and Bc5are connected in triple-tandem and only located in the 5′ -UTR of the cspABCDE mRNA in some B. cereus and B. thuringiensis

References