Difference between revisions of "Part:BBa K1954000"
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This entry is the open reading frame for the mutacin 1140 prepropeptide coded for by the ''lanA'' gene in ''Streptococcus mutans'' JH1140. Mutacin 1140 is type A lantibiotic (a linear lanthionine-containing antimicrobial peptide) differing from other bacteriocins by the presence of distinct post-translational modifications.  
 
This entry is the open reading frame for the mutacin 1140 prepropeptide coded for by the ''lanA'' gene in ''Streptococcus mutans'' JH1140. Mutacin 1140 is type A lantibiotic (a linear lanthionine-containing antimicrobial peptide) differing from other bacteriocins by the presence of distinct post-translational modifications.  
  
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The mature mutacin peptide is a 22 amino acid screw-shaped molecule that interferes with the viability of a broad range of Gram-positive bacteria by means of membrane perturbation. In the host organism. 10 of the 22 amino acids are modified to lanthionine, methyllanthionine, 2,3-didehydroalanine, 2,3-didehydrobutyrine and possibly S-(2-aminovinyl)-D-cysteine. In order for mutacin 1140 to be functional the action of modifying enzymes is required. These enzymes catalyze the formation of stereospecific thioether bridges, dehydration, decarboxylation of the core peptide and leader peptide cleavage, as well as act as exporters of the modified mutacin.
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The mature mutacin peptide is a 22 amino acid screw-shaped molecule that interferes with the viability of a broad range of Gram-positive bacteria by means of membrane perturbation leading to membrane disintegration. In order for mutacin 1140 to be functional the action of modifying enzymes is required. These enzymes catalyze the formation of stereospecific thioether bridges, dehydration, decarboxylation of the core peptide and leader peptide cleavage, as well as act as exporters of the modified mutacin. In the host organism, 10 of the 22 amino acids are modified to lanthionine, methyllanthionine, 2,3-didehydroalanine, 2,3-didehydrobutyrine and possibly S-(2-aminovinyl)-D-cysteine.  
 
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<partinfo>BBa_K1954000 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1954000 SequenceAndFeatures</partinfo>

Revision as of 13:55, 12 September 2016

LanA

This entry is the open reading frame for the mutacin 1140 prepropeptide coded for by the lanA gene in Streptococcus mutans JH1140. Mutacin 1140 is type A lantibiotic (a linear lanthionine-containing antimicrobial peptide) differing from other bacteriocins by the presence of distinct post-translational modifications.

The mature mutacin peptide is a 22 amino acid screw-shaped molecule that interferes with the viability of a broad range of Gram-positive bacteria by means of membrane perturbation leading to membrane disintegration. In order for mutacin 1140 to be functional the action of modifying enzymes is required. These enzymes catalyze the formation of stereospecific thioether bridges, dehydration, decarboxylation of the core peptide and leader peptide cleavage, as well as act as exporters of the modified mutacin. In the host organism, 10 of the 22 amino acids are modified to lanthionine, methyllanthionine, 2,3-didehydroalanine, 2,3-didehydrobutyrine and possibly S-(2-aminovinyl)-D-cysteine.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 57
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]