Difference between revisions of "Part:BBa K863006:Design"

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===Design Notes===
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==Design Notes==
 
Primers for isolation of the gene with BioBrick Prefix in the fwd primer and Suffix in the rev primer. <br>
 
Primers for isolation of the gene with BioBrick Prefix in the fwd primer and Suffix in the rev primer. <br>
 
fwd: 5'-ACGTGAATTCGCGGCCGCTTCTAGATGCAACGTCGTGATTTCTT-3' <br>
 
fwd: 5'-ACGTGAATTCGCGGCCGCTTCTAGATGCAACGTCGTGATTTCTT-3' <br>

Latest revision as of 09:10, 12 September 2016

ecol laccase from E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 225
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primers for isolation of the gene with BioBrick Prefix in the fwd primer and Suffix in the rev primer.
fwd: 5'-ACGTGAATTCGCGGCCGCTTCTAGATGCAACGTCGTGATTTCTT-3'
rev: 5'-ACGTCTGCAGCGGCCGCTACTAGTATTATACCGTAAACCCTAACA-3'

Source

The gene sequence was isolated from E. coli BL21(DE3).

References

Zeng, J., X. Lin, et al. (2011). "Oxidation of polycyclic aromatic hydrocarbons by the bacterial laccase CueO from E. coli." Appl Microbiol Biotechnol 89(6): 1841-1849.