Difference between revisions of "Part:pSB2K4"

Latest revision as of 19:48, 29 May 2007

pSB2K3-derived BioBrick plasmid free of many restriction sites

Derived from pSB2K3 with many restriction enzyme sites removed by site-directed mutagenesis (~2 dozen mutations). Plasmid is useful when there is a need to cut with a variety of different restriction enzymes. Most symmetric recognition sites have been removed, and some non-symmetric sites have also been removed including AarI, AcuI, BbsI, BbvCI, BciVI, BsaI, BsmBI, EarI, N.BstNBI, and SgrAI. Works in exactly same way as pSB2K3. See pSB2K3 for more information. Note that the VF2 site has a single base mutation in the middle of it. However, VF2 works well as a primer for PCR or sequencing with this plasmid.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 4446
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 4452
21
INCOMPATIBLE WITH RFC[21]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 4446
Illegal BglII site found at 1645
Illegal BglII site found at 2692
Illegal BamHI site found at 2566
23
INCOMPATIBLE WITH RFC[23]
Illegal prefix found at 4446
Illegal suffix found at 2
25
INCOMPATIBLE WITH RFC[25]
Illegal prefix found at 4446
Plasmid lacks a suffix.
Illegal XbaI site found at 4461
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal AgeI site found at 23
1000
INCOMPATIBLE WITH RFC[1000]
Plasmid lacks a prefix.
Plasmid lacks a suffix.

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>pSB2K4 short</partinfo>
-Derived from pSB2K3 with many restriction enzyme sites removed by site-directed mutagenesis (~2 dozen mutations). Plasmid is useful when there is a need to cut with a variety of different restriction enzymes. Most symmetric cutters have been removed, and some non-symmetric cutters have also including AarI, AcuI, BbsI, BbvCI, BciVI, BsaI, BsmBI, EarI, N.BstNBI, and SgrAI. Works in exactly same way as pSB2K3. See <bbpart>pSB2K3</bbpart> for more information. Note that the VF2 site has a single base mutation in the middle of it. However, VF2 has been used successfully as a primer for PCR with this plasmid.
+Derived from pSB2K3 with many restriction enzyme sites removed by site-directed mutagenesis (~2 dozen mutations). Plasmid is useful when there is a need to cut with a variety of different restriction enzymes. Most symmetric recognition sites have been removed, and some non-symmetric sites have also been removed including AarI, AcuI, BbsI, BbvCI, BciVI, BsaI, BsmBI, EarI, N.BstNBI, and SgrAI. Works in exactly same way as pSB2K3. See <bbpart>pSB2K3</bbpart> for more information. Note that the VF2 site has a single base mutation in the middle of it. However, VF2 works well as a primer for PCR or sequencing with this plasmid.
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