Difference between revisions of "Part:BBa K1895002"

Latest revision as of 09:50, 6 September 2016

dnaK Promoter

This is the dnaK promoter from E. coli K-12.

Usage and Biology

DnaK is a heat shock protein. It's role is that of a protein chaperone, it binds to proteins that have misfolded due to the stress. These proteins are then refolded in an HSP-mediated process or degraded (Arsène F, et al., 2000). Like other members of the Hsp70 family, dnaK in E. coli is upregulated by heat stress (Ritossa, F., 1996). Consequently its promoter must be stronger during heat shock to allow for overexpression. Although DnaK itself acts to shut off the heat shock response (Tilly, K. et al., 1983) we feel its promoter is a viable alternative heat shock promoter to the current registry entry, htpG (BBa_J45504).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K1895002 short</partinfo>
-This is the dnaK promoter
+This is the dnaK promoter from E. coli K-12.
-<!-- Add more about the biology of this part here
+<!-- Add more about the biology of this part here -->
 ===Usage and Biology===
+DnaK is a heat shock protein. It's role is that of a protein chaperone, it binds to proteins that have misfolded due to the stress. These proteins are then refolded in an HSP-mediated process or degraded (Arsène F, et al., 2000). Like other members of the Hsp70 family, dnaK in E. coli is upregulated by heat stress (Ritossa, F., 1996). Consequently its promoter must be stronger during heat shock to allow for overexpression. Although DnaK itself acts to shut off the heat shock response (Tilly, K. et al., 1983) we feel its promoter is a viable alternative heat shock promoter to the current registry entry, htpG ([https://parts.igem.org/Part:BBa_J45504 BBa_J45504]).
 <!-- -->