Difference between revisions of "Part:BBa K2012002:Experience"
| Line 5: | Line 5: | ||
===Applications of BBa_K2012002=== | ===Applications of BBa_K2012002=== | ||
| − | https:// | + | https://static.igem.org/mediawiki/parts/3/3d/Congo_red_stain.png |
Congo red staining<br> | Congo red staining<br> | ||
Latest revision as of 07:39, 5 September 2016
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K2012002
Congo red staining
For demonstrating expression of pleD, we used Congo red staining assay. As previous mentioned, high concentration of c-di-GMP could induce E. coli synthesize exopolysaccharides, and Congo red binding is a complex phenotype that reflects various outer membrane and surface properties including the presence of adhesive structures such as curli fimbria which are involved in biofilm formation. Wild type: DE3 contain pET28b plasmid, colony which was stained red color contain pET-pleD plasmid.
User Reviews
UNIQ1263764b2d99c45b-partinfo-00000000-QINU UNIQ1263764b2d99c45b-partinfo-00000001-QINU