Difference between revisions of "Part:BBa K2012002:Experience"
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===Applications of BBa_K2012002=== | ===Applications of BBa_K2012002=== | ||
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| + | Congo red staining | ||
| + | For demonstrating expression of pleD, we used Congo red staining assay. As previous mentioned, high concentration of c-di-GMP could induce E. coli synthesize exopolysaccharides, and Congo red binding is a complex phenotype that reflects various outer membrane and surface properties including the presence of adhesive structures such as curli fimbria which are involved in biofilm formation. Wild type: DE3 contain pET28b plasmid, colony which was stained red color contain pET-pleD plasmid. | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 06:51, 5 September 2016
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Applications of BBa_K2012002
Congo red staining For demonstrating expression of pleD, we used Congo red staining assay. As previous mentioned, high concentration of c-di-GMP could induce E. coli synthesize exopolysaccharides, and Congo red binding is a complex phenotype that reflects various outer membrane and surface properties including the presence of adhesive structures such as curli fimbria which are involved in biofilm formation. Wild type: DE3 contain pET28b plasmid, colony which was stained red color contain pET-pleD plasmid.
User Reviews
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