Difference between revisions of "Part:BBa K2042002"

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This is the coding sequence for a Polyhydroxyalkanoate synthase from Pseudomonas sp. MBEL 6-19. The enzyme takes the monomer (D)-lactyl-CoA and creates the PLA homopolymer. This sequence includes four mutations which were found to increase yields of PLA (Yang et al.)
 
This is the coding sequence for a Polyhydroxyalkanoate synthase from Pseudomonas sp. MBEL 6-19. The enzyme takes the monomer (D)-lactyl-CoA and creates the PLA homopolymer. This sequence includes four mutations which were found to increase yields of PLA (Yang et al.)
The sequence is codon optimized for Pseudomonas putida
 
 
  
 
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Revision as of 16:56, 4 September 2016


Pseudomonas sp. MBEL 6-19 PHA synthase 1

This is the coding sequence for a Polyhydroxyalkanoate synthase from Pseudomonas sp. MBEL 6-19. The enzyme takes the monomer (D)-lactyl-CoA and creates the PLA homopolymer. This sequence includes four mutations which were found to increase yields of PLA (Yang et al.)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1060
    Illegal NgoMIV site found at 1339
    Illegal AgeI site found at 135
    Illegal AgeI site found at 448
  • 1000
    COMPATIBLE WITH RFC[1000]