Difference between revisions of "Part:BBa K2042001"
RATOVOMANANA (Talk | contribs) |
RATOVOMANANA (Talk | contribs) |
||
Line 2: | Line 2: | ||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K2042001 short</partinfo> | <partinfo>BBa_K2042001 short</partinfo> | ||
+ | |||
This is the coding sequence for a class II poly(R)-hydroxyalkanoic acid (PHA) synthase 1 from Pseudomonas resinovorans. The enzyme takes the monomer (D)-lactyl-CoA and produce the PLA homopolymer. | This is the coding sequence for a class II poly(R)-hydroxyalkanoic acid (PHA) synthase 1 from Pseudomonas resinovorans. The enzyme takes the monomer (D)-lactyl-CoA and produce the PLA homopolymer. |
Revision as of 15:35, 4 September 2016
Pseudomonas resinovorans PHA synthase 1
This is the coding sequence for a class II poly(R)-hydroxyalkanoic acid (PHA) synthase 1 from Pseudomonas resinovorans. The enzyme takes the monomer (D)-lactyl-CoA and produce the PLA homopolymer.
This sequence is codon optimized for Pseudomonas putida and includes four amino acid mutations (E130D/S325T/S477G/Q481K) which were found to increase yields of PLA.
This type II PHA synthase 1 accept short-chain-length hydroxyacyl-CoAs including lactyl-CoA and 3-hydroxybutyryl-CoA as substrates and support the synthesis of P(3HB-co-LA).
This biobrick takes over Yale 2013 BBa_K1211002 biobrick. Indeed according to "http://www.ncbi.nlm.nih.gov/pubmed/21221571" the mutation on serine 477 is needed for PLA polymerization but in BBa_K1211002 this mutation is found on serine 475.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 87
Illegal XhoI site found at 172
Illegal XhoI site found at 1030 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1060
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 796