Difference between revisions of "Part:BBa K2030001"
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The upstream regulatory sequence to the gene <i>PCK1</i>, coding for Phosphoenolpyruvate carboxykinase. This promoter is induced by glycerol, acetate and ethanol (10x) [1].  
 
The upstream regulatory sequence to the gene <i>PCK1</i>, coding for Phosphoenolpyruvate carboxykinase. This promoter is induced by glycerol, acetate and ethanol (10x) [1].  
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After sequencing of this part, it can be noted that the sequence differs in 3 positions when comparing against the reference genome (S228c). However, it matches perfectly with the genomic sequence of [http://www.yeastgenome.org/contig/JRIV01000108.1/overview CEN.PK2] where the promoter was amplified from. 
  
 
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Revision as of 07:31, 31 August 2016


pPCK1 S. cerevisiae promoter

The upstream regulatory sequence to the gene PCK1, coding for Phosphoenolpyruvate carboxykinase. This promoter is induced by glycerol, acetate and ethanol (10x) [1].

After sequencing of this part, it can be noted that the sequence differs in 3 positions when comparing against the reference genome (S228c). However, it matches perfectly with the genomic sequence of [http://www.yeastgenome.org/contig/JRIV01000108.1/overview CEN.PK2] where the promoter was amplified from.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 221
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 364
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1] K. Weinhandl, M. Winkler, A. Glieder, and A. Camattari, “Carbon source dependent promoters in yeasts,” Microbial Cell Factories, vol. 13, no. 1, 2014.