Difference between revisions of "Part:BBa K2042000:Design"
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===References=== | ===References=== | ||
| − | Biosynthesis of polylactic acid and its copolymers using evolved propionate CoA transferase and PHA synthase. <br | + | Biosynthesis of polylactic acid and its copolymers using evolved propionate CoA transferase and PHA synthase. <br> |
Yang TH1, Kim TW, Kang HO, Lee SH, Lee EJ, Lim SC, Oh SO, Song AJ, Park SJ, Lee SY. | Yang TH1, Kim TW, Kang HO, Lee SH, Lee EJ, Lim SC, Oh SO, Song AJ, Park SJ, Lee SY. | ||
| − | Microbial production of lactate-containing polyesters. <br | + | Microbial production of lactate-containing polyesters. <br> |
Yang JE1, Choi SY, Shin JH, Park SJ, Lee SY. | Yang JE1, Choi SY, Shin JH, Park SJ, Lee SY. | ||
Revision as of 20:43, 29 August 2016
Propionate CoA transferase for P. putida
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 675
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1081
Illegal BsaI site found at 1552
Design Notes
The iGEM Team Evry 2016 used this enzyme as the first enzyme in the pathway to produce Polylactic acid (PLA) in Pseudomonas putida.
Source
Synthetic synthesis
References
Biosynthesis of polylactic acid and its copolymers using evolved propionate CoA transferase and PHA synthase.
Yang TH1, Kim TW, Kang HO, Lee SH, Lee EJ, Lim SC, Oh SO, Song AJ, Park SJ, Lee SY.
Microbial production of lactate-containing polyesters.
Yang JE1, Choi SY, Shin JH, Park SJ, Lee SY.