Difference between revisions of "Part:BBa K2077000:Experience"

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===Applications of BBa_K2077000===
 
===Applications of BBa_K2077000===
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<p>[[File:T--RHIT--Psb416GPDTuMasterPlate2.png|200px|thumb|left|alt text]][[File:T--RHIT--KnockOutInitialTest.png|200px|thumb|left|alt text]]</p>  
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[[File:T--RHIT--Psb416GPDTuMasterPlate2.png|200px|thumb|left|Figure 1.  BY4741 yeast transformants with pSB416 GPD with mRPS12 TU as insert plated on CSM-URA and grown for five days.]]
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[[File:T--RHIT--KnockOutInitialTest.png|200px|thumb|left|Figure 2.  BY4741 yeast without pSB416-GPD plated on CSM-URA and CSM-His media.  Picture was taken after five days of incubation.]]   
  
  
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We have verified the function of the selectable markers, the promoter, the terminator, and the biobrick prefix and suffix in pSB416-GPD. By4741 transformed with pSB-416 GPD regained the ability to grow on CSM—URA as shown in Figure 1 & 2. Figure 2 demonstrates the function of the promoter, terminator and the biobrick prefix and suffix in pSB416-GPD. The yeast on the bottom row of the figure were transformed with pSB416-GPD with mls-yeGEP inserted into the vector using the bio brick prefix and suffix. The fluorescence demonstrated by the yeast verifies the function of the promoter and terminator in the vector.
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We have verified the function of the selectable markers, the promoter, the terminator, and the biobrick prefix and suffix in pSB416-GPD. BY4741 yeast that have their URA3 and HIS genes knocked out transformed with pSB416-GPD regained the ability to grow on CSM—URA as shown in Figures 1 & 2. Figure 3 demonstrates the function of the promoter, terminator and the biobrick prefix and suffix in pSB416-GPD. The yeast on the bottom row of the figure were transformed with pSB416-GPD with mls-yeGEP inserted into the vector using the bio brick prefix and suffix. The fluorescence demonstrated by the yeast verifies the function of the promoter and terminator in the vector.
  
 
===User Reviews===
 
===User Reviews===

Revision as of 00:56, 11 August 2016


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2077000

Figure 1. BY4741 yeast transformants with pSB416 GPD with mRPS12 TU as insert plated on CSM-URA and grown for five days.
Figure 2. BY4741 yeast without pSB416-GPD plated on CSM-URA and CSM-His media. Picture was taken after five days of incubation.


Figure 3. Top Left: BY4147 under fluorescent light. Top Right BY4147 under Normal light. Bottom Left: BY4741 with insert under fluorescent light. Bottom Right: BY4741 with insert under normal light


We have verified the function of the selectable markers, the promoter, the terminator, and the biobrick prefix and suffix in pSB416-GPD. BY4741 yeast that have their URA3 and HIS genes knocked out transformed with pSB416-GPD regained the ability to grow on CSM—URA as shown in Figures 1 & 2. Figure 3 demonstrates the function of the promoter, terminator and the biobrick prefix and suffix in pSB416-GPD. The yeast on the bottom row of the figure were transformed with pSB416-GPD with mls-yeGEP inserted into the vector using the bio brick prefix and suffix. The fluorescence demonstrated by the yeast verifies the function of the promoter and terminator in the vector.

User Reviews

UNIQ272fd13d1f65610a-partinfo-00000000-QINU UNIQ272fd13d1f65610a-partinfo-00000001-QINU