Difference between revisions of "Part:BBa K2077000:Experience"

Revision as of 22:47, 9 August 2016

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Please enter how you used this part and how it worked out.

Applications of BBa_K2077000

alt text

Figure 3. Top Left: BY4147 under fluorescent light. Top Right BY4147 under Normal light. Bottom Left: BY4741 with insert under fluorescent light. Bottom Right: BY4741 with insert under normal light

We have verified the function of the selectable markers, the promoter, the terminator, and the biobrick prefix and suffix in pSB416-GPD. By4741 transformed with pSB-416 GPD regained the ability to grow on CSM—URA as shown in Figure 1 & 2. Figure 2 demonstrates the function of the promoter, terminator and the biobrick prefix and suffix in pSB416-GPD. The yeast on the bottom row of the figure were transformed with pSB416-GPD with mls-yeGEP inserted into the vector using the bio brick prefix and suffix. The fluorescence demonstrated by the yeast verifies the function of the promoter and terminator in the vector.

User Reviews

UNIQ49fcc4043cccdfb3-partinfo-00000000-QINU UNIQ49fcc4043cccdfb3-partinfo-00000001-QINU

@@ Line 5: / Line 5: @@
 ===Applications of BBa_K2077000===
-<p>[[File:T--RHIT--FluorsenceComparisonpicture.PNG]]</p>
+<p>[[File:T--RHIT--Psb416GPDTuMasterPlate2.png|200px|thumb|left|alt text]][[File:T--RHIT--KnockOutInitialTest.png|200px|thumb|left|alt text]]</p>
-Figure 2. Top Left: By4147 under flourscent light. Top Right By4147 under Normal light. Bottom Left: By 4741 with insert under flourscent light. Bottom Right: By4741 with insert under normal light
+[[File:T--RHIT--FluorsenceComparisonpicture.PNG|200px|thumb|left|Figure 3. Top Left: BY4147 under fluorescent light. Top Right BY4147 under Normal light. Bottom Left: BY4741 with insert under fluorescent light. Bottom Right: BY4741 with insert under normal light]]
-We have verified the function of the selectable markers, the promoter, the terminator, and the biobrick prefix and suffix in pSB416-GPD. By4741 transformed with PBS 416 GPD regained the ability to grow on CSM—URA. Figure 2 demonstrates the function of the promoter, terminator and the biobrick prefix and suffix in pSB416-GPD. The yeast on the bottom row of the figure were transformed with pSB416 GPD with mls-yeGEP inserted into the vector using the bio brick prefix and suffix. The fluorescence demonstrated by the yeast verifies the function of the promoter and terminator in the vector.
+We have verified the function of the selectable markers, the promoter, the terminator, and the biobrick prefix and suffix in pSB416-GPD. By4741 transformed with pSB-416 GPD regained the ability to grow on CSM—URA as shown in Figure 1 & 2. Figure 2 demonstrates the function of the promoter, terminator and the biobrick prefix and suffix in pSB416-GPD. The yeast on the bottom row of the figure were transformed with pSB416-GPD with mls-yeGEP inserted into the vector using the bio brick prefix and suffix. The fluorescence demonstrated by the yeast verifies the function of the promoter and terminator in the vector.
 ===User Reviews===