Difference between revisions of "Part:BBa K2180009"

 
m
Line 3: Line 3:
 
<partinfo>BBa_K2180009 short</partinfo>
 
<partinfo>BBa_K2180009 short</partinfo>
  
−
This is the assembly of our basic parts BBa_K2180004 , BBa_K2180007 without the Fur Box and BBa_K2180008
+
This is the assembly of our basic parts BBa_K2180004 , BBa_K2180007 and BBa_K2180008
−
We delete the Fur Box in order to use it like a normal promoter.
+
 
The NcoI restriction site is overlapping parts BBa_K2180004 and BBa_K2180007 at the Start codon of LacI + LVA.
 
The NcoI restriction site is overlapping parts BBa_K2180004 and BBa_K2180007 at the Start codon of LacI + LVA.
  

Revision as of 20:23, 5 August 2016


LacI-LVA for B. subtilis

This is the assembly of our basic parts BBa_K2180004 , BBa_K2180007 and BBa_K2180008 The NcoI restriction site is overlapping parts BBa_K2180004 and BBa_K2180007 at the Start codon of LacI + LVA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 178
    Illegal AgeI site found at 966
  • 1000
    COMPATIBLE WITH RFC[1000]