Difference between revisions of "Part:BBa J100281"

 
 
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<partinfo>BBa_J100281 short</partinfo>
 
<partinfo>BBa_J100281 short</partinfo>
  
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Fusion protein of tetA and sacB connected with a linker sequence, with a pBAD promotor and RBS. TetA encodes tetracycline resistance and sacB kills E. coli in the presence of sucrose, allowing for both positive and negative selection. pBAD is an arabinose-inducible promoter.  
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Fusion protein of tetA and sacB connected with a linker sequence, with a pBAD promotor. TetA encodes tetracycline resistance and sacB kills E. coli in the presence of sucrose, allowing for both positive and negative selection. RBS was deleted in template. Fixed version of part is J100286. pBAD is an arabinose-inducible promoter.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 18:53, 8 July 2016


tetA+sacB without RBS

Fusion protein of tetA and sacB connected with a linker sequence, with a pBAD promotor. TetA encodes tetracycline resistance and sacB kills E. coli in the presence of sucrose, allowing for both positive and negative selection. RBS was deleted in template. Fixed version of part is J100286. pBAD is an arabinose-inducible promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
    Illegal NheI site found at 280
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
    Illegal BamHI site found at 426
    Illegal BamHI site found at 2578
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 452
    Illegal NgoMIV site found at 820
    Illegal NgoMIV site found at 980
    Illegal AgeI site found at 1322
    Illegal AgeI site found at 2525
    Illegal AgeI site found at 2552
  • 1000
    COMPATIBLE WITH RFC[1000]