Difference between revisions of "Part:BBa J100279"

 
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<partinfo>BBa_J100279 short</partinfo>
 
<partinfo>BBa_J100279 short</partinfo>
  
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The BD10 C Dog Ribosome Binding Site has a lower binding efficiency than BD18. PCR and Golden Gate Assembly was used to cut out the BD18 C Dog RBS of part J100275 and insert in the BD10 C Dog RBS.
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The BD10 C Dog Ribosome Binding Site has a lower binding efficiency than BD18. PCR and Golden Gate Assembly was used to cut out the BD18 C Dog RBS of part J100275 and insert in the BD10 C Dog RBS. ''J100279'' is an improvement over [[Part:BBa_J100255]] based on [[Part:BBa_J119367]].  
  
  

Revision as of 15:32, 8 July 2016


tClone Tet+PDB riboswitch with P9 and BD10

The BD10 C Dog Ribosome Binding Site has a lower binding efficiency than BD18. PCR and Golden Gate Assembly was used to cut out the BD18 C Dog RBS of part J100275 and insert in the BD10 C Dog RBS. J100279 is an improvement over Part:BBa_J100255 based on Part:BBa_J119367.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 350
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 496
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 522
    Illegal NgoMIV site found at 890
    Illegal NgoMIV site found at 1050
  • 1000
    COMPATIBLE WITH RFC[1000]