Difference between revisions of "Part:BBa J100268"

 
 
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<partinfo>BBa_J100268 short</partinfo>
 
<partinfo>BBa_J100268 short</partinfo>
  
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In their 2014 paper "Negative selection and stringency modulation in phage-assisted continuous evolution," Carlson et al., designed part J100268 to be used as a drift plasmid in phage-assisted continuous evolution (PACE).
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In their 2014 paper "[http://www.nature.com/nchembio/journal/v10/n3/full/nchembio.1453.html Negative selection and stringency modulation in phage-assisted continuous evolution]," Carlson ''et al.'', designed part J100268 to be used as an accessory plasmid in phage-assisted continuous evolution (PACE). This DNA part comes from a collection of 8 plasmids numbers J100264 - J100271. PACE employs M13 phage and ''E. coli'' in a chemostat.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 21:00, 17 June 2016


pJC184d5

In their 2014 paper "[http://www.nature.com/nchembio/journal/v10/n3/full/nchembio.1453.html Negative selection and stringency modulation in phage-assisted continuous evolution]," Carlson et al., designed part J100268 to be used as an accessory plasmid in phage-assisted continuous evolution (PACE). This DNA part comes from a collection of 8 plasmids numbers J100264 - J100271. PACE employs M13 phage and E. coli in a chemostat.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2877
    Illegal EcoRI site found at 5360
    Illegal XbaI site found at 7731
    Illegal PstI site found at 4814
    Illegal PstI site found at 6294
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2877
    Illegal EcoRI site found at 5360
    Illegal PstI site found at 4814
    Illegal PstI site found at 6294
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2877
    Illegal EcoRI site found at 5360
    Illegal BglII site found at 3415
    Illegal BglII site found at 6176
    Illegal BamHI site found at 2024
    Illegal BamHI site found at 2293
    Illegal BamHI site found at 4434
    Illegal BamHI site found at 6963
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2877
    Illegal EcoRI site found at 5360
    Illegal XbaI site found at 7731
    Illegal PstI site found at 4814
    Illegal PstI site found at 6294
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2877
    Illegal EcoRI site found at 5360
    Illegal XbaI site found at 7731
    Illegal PstI site found at 4814
    Illegal PstI site found at 6294
    Illegal AgeI site found at 1859
    Illegal AgeI site found at 2165
    Illegal AgeI site found at 2654
    Illegal AgeI site found at 2852
    Illegal AgeI site found at 3313
    Illegal AgeI site found at 3536
    Illegal AgeI site found at 4709
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5828
    Illegal SapI site found at 1841
    Illegal SapI.rc site found at 3503