Difference between revisions of "Part:BBa J100264"

Latest revision as of 20:56, 17 June 2016

pJC156c2 (Carlson et al. 2014)

In their 2014 paper "[http://www.nature.com/nchembio/journal/v10/n3/full/nchembio.1453.html Negative selection and stringency modulation in phage-assisted continuous evolution]," Carlson et al., designed part J100264 when developing geneIII neg, the dominant negative allele of geneIII of the M13 phage. This DNA part comes from a collection of 8 plasmids numbers J100264 - J100271. They are used in Phage Assisted Continuous Evolution (PACE) which employs M13 phage and E. coli in a chemostat.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 1527
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 1527
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 1527
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 1527
Illegal NgoMIV site found at 2803
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 2518
Illegal SapI.rc site found at 767

@@ Line 3: / Line 3: @@
 <partinfo>BBa_J100264 short</partinfo>
-In their 2014 paper "[http://www.nature.com/nchembio/journal/v10/n3/full/nchembio.1453.html Negative selection and stringency modulation in phage-assisted continuous evolution]," Carlson et al., designed part J100264 when developing geneIII neg, the dominant negative allele of geneIII of the M13 phage.
+In their 2014 paper "[http://www.nature.com/nchembio/journal/v10/n3/full/nchembio.1453.html Negative selection and stringency modulation in phage-assisted continuous evolution]," Carlson ''et al.'', designed part J100264 when developing geneIII neg, the dominant negative allele of geneIII of the M13 phage. This DNA part comes from a collection of 8 plasmids numbers J100264 - J100271. They are used in Phage Assisted Continuous Evolution (PACE) which employs M13 phage and E. coli in a chemostat.
 <!-- Add more about the biology of this part here