Difference between revisions of "Part:BBa J100264"

 
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<partinfo>BBa_J100264 short</partinfo>
 
<partinfo>BBa_J100264 short</partinfo>
  
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In their 2014 paper "Negative selection and stringency modulation in phage-assisted continuous evolution," Carlson et al., designed part J100264 when developing geneIII neg, the dominant negative allele of geneIII of the M13 phage.  
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In their 2014 paper "[http://www.nature.com/nchembio/journal/v10/n3/full/nchembio.1453.html Negative selection and stringency modulation in phage-assisted continuous evolution]," Carlson et al., designed part J100264 when developing geneIII neg, the dominant negative allele of geneIII of the M13 phage.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 20:53, 17 June 2016


pJC156c2 (Carlson et al. 2014)

In their 2014 paper "[http://www.nature.com/nchembio/journal/v10/n3/full/nchembio.1453.html Negative selection and stringency modulation in phage-assisted continuous evolution]," Carlson et al., designed part J100264 when developing geneIII neg, the dominant negative allele of geneIII of the M13 phage.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1527
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1527
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1527
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1527
    Illegal NgoMIV site found at 2803
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2518
    Illegal SapI.rc site found at 767