Difference between revisions of "Part:BBa P10500"

 
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<partinfo>BBa_P10500 short</partinfo>
 
<partinfo>BBa_P10500 short</partinfo>
  
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This is the universal acceptor plasmid for creating new PhytoBricks parts. This sequence is contained within the BioBricks vector pSB1C3.
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This is the universal acceptor plasmid for creating new PhytoBricks parts. It contains the lacZ-alpha fragment in the cloning site for blue-white screening through the use of IPTG and X-Gal on the agar plates.
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This sequence is contained within the BioBricks vector pSB1C3.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 19:01, 10 June 2016


PhytoBricks Universal Acceptor

This is the universal acceptor plasmid for creating new PhytoBricks parts. It contains the lacZ-alpha fragment in the cloning site for blue-white screening through the use of IPTG and X-Gal on the agar plates.

This sequence is contained within the BioBricks vector pSB1C3.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 247
    Illegal XbaI site found at 220
    Illegal PstI site found at 208
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 247
    Illegal PstI site found at 208
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 247
    Illegal BamHI site found at 226
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 247
    Illegal XbaI site found at 220
    Illegal PstI site found at 208
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 247
    Illegal XbaI site found at 220
    Illegal PstI site found at 208
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 598