Difference between revisions of "Part:BBa K1904005"

Latest revision as of 03:48, 8 June 2016

Fused protein (Holin/Endolysin) with RBS and terminator, C-terminal HisTag, (RFC 25 N-Part).

This part contains the coding sequences of BBa_K1904002 and BBa_K1904004 which consist of an specific endolysin for Acinetobacter baumannii and a T4 holin, forming a fusion protein. Additionally the part is assembled with B0034 (RBS) and B0015 (double terminator). This intermediate biobrick should be cloned into a promoter-containing plasmid for its usage. The standard of the biobrick is RFC 25, but as it does not tolerate changes in the N-part of the protein it is designed as an Freiburg N-part. Ultimately, a C-terminal HisTag was added in order to allow its purification via a Nickel-affinity chromatography.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K1904005 short</partinfo>
-This part contains the coding sequences of BBa_K1904002 and BBa_K1904004 which consist of an specific endolysin for Acinetobacter baumannii and a T4 holin, forming a fusion protein. Additionally the part is assembled with B0034 (RBS) and B0015 (double terminator). This intermediate biobrick should be cloned into a promoter-containing plasmid for its usage. The standard of the biobrick is RFC 25, but as it does not tolerate changes in the N-part of the protein it is designed as an Freiburg N-part. Ultimately a C-terminal HisTag was added in order to allow its purification via a Nickel-affinity chromatography.
+This part contains the coding sequences of BBa_K1904002 and BBa_K1904004 which consist of an specific endolysin for ''Acinetobacter baumannii'' and a T4 holin, forming a fusion protein. Additionally the part is assembled with B0034 (RBS) and B0015 (double terminator). This intermediate biobrick should be cloned into a promoter-containing plasmid for its usage. The standard of the biobrick is RFC 25, but as it does not tolerate changes in the N-part of the protein it is designed as an Freiburg N-part. Ultimately, a C-terminal HisTag was added in order to allow its purification via a Nickel-affinity chromatography.
 <!-- Add more about the biology of this part here