Difference between revisions of "Part:BBa K1904003"

Revision as of 03:41, 8 June 2016

T4 Holin with RBS and terminator.

This biobrick contains the coding sequence for a T4 Holin (BBa_K112805) which promotes pore formation on inner membrane of bacteria allowing lysozyme to reach periplasm and degrade peptidoglycan layer. The part, designed in RFC10, was assembled with B0034 (RBS) and B0015 (double terminator). This intermediate biobrick should be cloned into a promoter-containing plasmid for its usage.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K1904003 short</partinfo>
-This biobrick contains the coding sequence for a T4 Holin (BBa_K112805) which form pores on inner membrane of bacteria allowing lysozyme to reach periplasm and degrade peptidoglycan layer. The part is assembled with B0034 (RBS) and B0015 (double terminator). This intermediate biobrick should be cloned into a promoter-containing plasmid for its usage.
+This biobrick contains the coding sequence for a T4 Holin (BBa_K112805) which promotes pore formation on inner membrane of bacteria allowing lysozyme to reach periplasm and degrade peptidoglycan layer. The part, designed in RFC10, was assembled with B0034 (RBS) and B0015 (double terminator). This intermediate biobrick should be cloned into a promoter-containing plasmid for its usage.
 <!-- Add more about the biology of this part here