Difference between revisions of "Part:BBa K1904002"
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<partinfo>BBa_K1904002 short</partinfo> | <partinfo>BBa_K1904002 short</partinfo> | ||
| − | This biobrick is | + | This biobrick is comprised by the mutated endolysin (BBa_K1904000) assembled with a double terminator (B0015); BBa_K1904002 is in RFC 25 standard to allow protein engineering. It is important to mention that for specifc iGEM TecCEM 16 project purposes, this BB was designed as a C-part for protein fusion. In order to use this part alone, it should be cloned into a plasmid containing promoter and RBS sequences. Additionally the part was complemented with a C-terminal HisTag in order to allow its purification via a Nickel-affinity chromatography. |
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Latest revision as of 03:28, 8 June 2016
Mutated endolysin specific for Acinetobacter baumannii with terminator. C-terminal HisTag
This biobrick is comprised by the mutated endolysin (BBa_K1904000) assembled with a double terminator (B0015); BBa_K1904002 is in RFC 25 standard to allow protein engineering. It is important to mention that for specifc iGEM TecCEM 16 project purposes, this BB was designed as a C-part for protein fusion. In order to use this part alone, it should be cloned into a plasmid containing promoter and RBS sequences. Additionally the part was complemented with a C-terminal HisTag in order to allow its purification via a Nickel-affinity chromatography.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]