Difference between revisions of "Part:BBa K1904001"
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<partinfo>BBa_K1904001 short</partinfo> | <partinfo>BBa_K1904001 short</partinfo> | ||
| − | This biobrick is comprised by the mutated endolysin (BBa_K1904000) assembled with B0034 (RBS) and B0015 (double terminator), all in RFC10 standard. This intermediate biobrick should be cloned into a promoter-containing plasmid for its usage. | + | This biobrick is comprised by the mutated endolysin (BBa_K1904000) assembled with B0034 (RBS) and B0015 (double terminator), all in RFC10 standard. This intermediate biobrick should be cloned into a promoter-containing plasmid for its usage. This part was complemented with a N-terminal HisTag in order to allow its purification via a Nickel-affinity chromatography. |
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Latest revision as of 02:58, 7 June 2016
Endolysin with RBS and terminator. N-terminal HisTag
This biobrick is comprised by the mutated endolysin (BBa_K1904000) assembled with B0034 (RBS) and B0015 (double terminator), all in RFC10 standard. This intermediate biobrick should be cloned into a promoter-containing plasmid for its usage. This part was complemented with a N-terminal HisTag in order to allow its purification via a Nickel-affinity chromatography.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]