Difference between revisions of "Help:2016 InterLab Measurement Kit"

Revision as of 18:55, 31 May 2016

Registry Help Pages:

TOC
At-a-Glance
FAQ

InterLab Measurement Kit

New for 2016, we are providing teams with the InterLab Measurement Kit! The InterLab activity is completely optional and is not required for any team.

Please note: there is a small error on the printed label on the box. There is one (1) FITC tube in the kit and NOT two (2).

Important Update: A few teams have reported that the tubes containing the DNA plasmids are empty. The tubes we used don't appear to be airtight. Please try resuspending the tubes in 10uLs of diH2O and vortexing them well, before spinning them down and transforming your cells.

The kit includes 7 tubes (5 with plasmid DNA and 3 with standard reagents):

Plasmid DNA (100 pg/uL in 10uL of Buffer EB)

Test Device 1: J23101.B0034.E0040.B0015 in pSB1C3
Test Device 2: J23106.B0034.E0040.B0015 in pSB1C3
Test Device 3: J23117.B0034.E0040.B0015 in pSB1C3
Positive Control Device: I20270 in pSB1C3
Negative Control Device: R0040 in pSB1C3

FITC Standard: one tube with dried down FITC for creating a FITC standard
LUDOX: one tube with 30% colloidal silica suspended in 1mL of water

For details on how to use this InterLab Measurement Kit, please check out the 2016 InterLab Page.
(URL: http://2016.igem.org/Tracks/Measurement/Interlab_study)

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-<b>Important Update:</b> A few teams have reported that the tubes containing the DNA plasmids are empty. The tubes we used don't appear to be airtight. Please try resuspending the tubes in 10uLs of diH2O and vortexing them well, before spinning them down and transforming your cells.
+<font color=purple><b>Important Update:</b> A few teams have reported that the tubes containing the DNA plasmids are empty. The tubes we used don't appear to be airtight. Please try resuspending the tubes in 10uLs of diH2O and vortexing them well, before spinning them down and transforming your cells.</font color>
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