Difference between revisions of "Help:IGEM 07 Parts Kit"
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The iGEM 2007 Parts Kit was shipped to teams this May.  The collection of four plates contains all parts available in the Registry as of mid-Spring 2007.
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The iGEM 2007 Parts Kit was shipped to teams this May.  The collection of four plates contains all parts available in the Registry as of mid-Spring 2007.  Here is what the kit looks like.
  
 
[[Image:FirstPlates.JPG|left|250px]]
 
[[Image:FirstPlates.JPG|left|250px]]
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==Storage==
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Store the DNA Parts Kit with the plastic lid and the adhesive foil cover in the -20C freezer.
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==Usage==
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The DNA Parts Kit is a tool available to you to perform synthetic biology with standard biological parts. It contains dry DNA of ALL of the parts that are available in the Registry up until May 2007. You will transform the DNA into cells and make your own glycerol stocks of any part that you wish. It does not contain enough DNA to do assembly.  To use the DNA in the Parts Kit follow the following instructions:
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:# Puncture a hole through the foil with a pipette tip into the well that corresponds to the Biobrick&trade; part that you want
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:# Add 15 uL of diH2O (deionized water)
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:# Take 1uL DNA and [http://openwetware.org/wiki/Bacterial_Transformation transform] into your desired competent cells, plate out on a plate with the correct antibiotic'''*''' and grow overnight.  Your goal here is to obtain single colonies. 
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:#Pick a single colony and inoculate broth (again, with the correct antibiotic) and grow ~18 hours.
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:#Use the resulting culture to miniprep the DNA AND make your own glycerol stock (for further instruction on making a glycerol see [http://openwetware.org/wiki/Endy:Making_a_long_term_stock_of_bacteria this page]).
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<small>'''*'''To know which antibiotics to use, look at the plasmid that the part is in.  The [https://parts.igem.org/wiki/index.php/Help:Plasmids/Nomenclature naming scheme] for plasmids is specifically designed to indicate antibiotic resistance.</small>

Revision as of 04:02, 26 May 2007

The iGEM 2007 Parts Kit was shipped to teams this May. The collection of four plates contains all parts available in the Registry as of mid-Spring 2007. Here is what the kit looks like.

FirstPlates.JPG


Storage

Store the DNA Parts Kit with the plastic lid and the adhesive foil cover in the -20C freezer.


Usage

The DNA Parts Kit is a tool available to you to perform synthetic biology with standard biological parts. It contains dry DNA of ALL of the parts that are available in the Registry up until May 2007. You will transform the DNA into cells and make your own glycerol stocks of any part that you wish. It does not contain enough DNA to do assembly. To use the DNA in the Parts Kit follow the following instructions:

  1. Puncture a hole through the foil with a pipette tip into the well that corresponds to the Biobrick™ part that you want
  2. Add 15 uL of diH2O (deionized water)
  3. Take 1uL DNA and [http://openwetware.org/wiki/Bacterial_Transformation transform] into your desired competent cells, plate out on a plate with the correct antibiotic* and grow overnight. Your goal here is to obtain single colonies.
  4. Pick a single colony and inoculate broth (again, with the correct antibiotic) and grow ~18 hours.
  5. Use the resulting culture to miniprep the DNA AND make your own glycerol stock (for further instruction on making a glycerol see [http://openwetware.org/wiki/Endy:Making_a_long_term_stock_of_bacteria this page]).

*To know which antibiotics to use, look at the plasmid that the part is in. The naming scheme for plasmids is specifically designed to indicate antibiotic resistance.