Difference between revisions of "RFC25 "Freiburg standard" parts"

(→‎Reptorter proteins)
(→‎Reptorter proteins)
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==Transcription Regulators==
 
==Transcription Regulators==
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{| class="wikitable"
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!colspan="6"|Fluorescent proteins
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|VP16
 
|VP16
 
|BBa_K1150001
 
|BBa_K1150001
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==Split proteins==
 
==Split proteins==
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{| class="wikitable"
 +
!colspan="6"|Fluorescent proteins
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|-
 
|TEV Protease
 
|TEV Protease
 
|BBa_K1159101
 
|BBa_K1159101
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|BBa_K863110
 
|BBa_K863110
 
|}
 
|}
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==Protein tags==
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==Affinity tags==
 
{| class="wikitable"
 
{| class="wikitable"
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!colspan="6"|Fluorescent proteins
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!colspan="6"|Affinity tags
 
|-
 
|-
 
|''Strep''-tag II an N-part
 
|''Strep''-tag II an N-part
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|BBa_K823034
 
|BBa_K823034
 
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<groupparts>iGEM07 Freiburg</groupparts>
 

Revision as of 00:11, 15 May 2016

Reptorter proteins

Fluorescent proteins

Fluorescent proteins
Protein Color BioBrick Excitation [nm] Emission [nm] Quantum Yield
Cerulean BBa_I757009
GFP Green BBa_K648013 484 507 0.60
GFP BBa_K863120
GFP BBa_K823039
sfGFP Green BBa_K1321337 485 510 0.65
Dronpa (photoactivatable) Green BBa_K1680006 503 518
mVenus BBa_I757008
sYFP2 Yellow BBa_K1159301 514 527 0.61
eCFP Cyan BBa_K1159302 439 476 0.40
mRFP BBa_K1351021 584 607
mRFP Red BBa_K1615089 584 607 0.25
mKate2 BBa_K823029 588 633

Values for the fluorescent proteins were taken from http://www.microscopyu.com/articles/livecellimaging/fpintro.html.

Luciferases

Luciferases
Protein BioBrick Size [kDa]
Firefly luciferase BBa_K525999 61
Renilla luciferase 36
NanoLuc luciferase BBa_K1159001 19
Gaussia luciferase

Transmembrane Domains

Linker domains

Linker
Protein Sequence BioBrick Length [residues]
Short Linker (Gly-Gly-Ser-Gly) BBa_K243004 4
Middle Linker 2x (Gly-Gly-Ser-Gly) BBa_K243005 8
Long Linker 3x (Gly-Gly-Ser-Gly) BBa_K243006 12

Catalytic domains

Fluorescent proteins
Erythromycin Esterase Type II BBa_K1159000
Laccase BBa_K1159002
Laccase TVEL5 BBa_K1615067
TEV Protease BBa_K1159100
Thermonuclease BBa_K1159105
Heroin Esterase BBa_K1615045
CRE recombinase BBa_K1680007
Monoamine Oxidase A BBa_K1615022
Endo-β-Galactosidase BBa_K1483001
α-Galactosidase BBa_K1483002
Acetylgalactosamindase BBa_K1483000
2-oxoglutarate oxygenase/decarboxylase BBa_K1065000

Transcription Regulators

Fluorescent proteins
VP16 BBa_K1150001

Split proteins

Fluorescent proteins
TEV Protease BBa_K1159101 BBa_K1159102
SpyCatcher/SpyTag BBa_K1159200 BBa_K1159201

Binding proteins

Fluorescent proteins
Fluorescein binding Anticalin BBa_K1159003
Digoxigenin binding Anticalin BBa_K243003
HER2 binding DARPin BBa_K404302
HER2 binding Affibody BBa_K404302
Nickel Binding Protein BBa_K1321009
Cellulose binding Domain BBa_K863110

Affinity tags

Affinity tags
Strep-tag II an N-part BBa_K549018
HA tag BBa_K1150016
His6-tag BBa_I757013
SpyTag BBa_K1159020
Strep-tag II BBa_J18916
cMyc-tag BBa_K823036
3x FLAG tag BBa_K823034