Difference between revisions of "RFC25 "Freiburg standard" parts"
VolkerMorath (Talk | contribs) (→Protein tags) |
VolkerMorath (Talk | contribs) (→Protein tags) |
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| Line 204: | Line 204: | ||
BBa_K1150016 | BBa_K1150016 | ||
| − | His<sub>6 | + | His<sub>6</sub>-tag |
BBa_I757013 | BBa_I757013 | ||
SpyTag | SpyTag | ||
BBa_K1159020 | BBa_K1159020 | ||
Revision as of 23:28, 14 May 2016
Contents
Reptorter proteins
Fluorescent proteins
| Fluorescent proteins | |||||
|---|---|---|---|---|---|
| Protein | Color | BioBrick | Excitation [nm] | Emission [nm] | Quantum Yield |
| GFP | Green | BBa_K648013 | 484 | 507 | 0.60 |
| sfGFP | Green | BBa_K1321337 | 485 | 510 | 0.65 |
| Dronpa (photoactivatable) | Green | BBa_K1680006 | 503 | 518 | |
| mVenus | BBa_I757008 | ||||
| sYFP2 | Yellow | BBa_K1159301 | 514 | 527 | 0.61 |
| eCFP | Cyan | BBa_K1159302 | 439 | 476 | 0.40 |
| mRFP | BBa_K1351021 | 584 | 607 | ||
| mRFP | Red | BBa_K1615089 | 584 | 607 | 0.25 |
Values for the fluorescent proteins were taken from http://www.microscopyu.com/articles/livecellimaging/fpintro.html.
Luciferases
| Luciferases | |||
|---|---|---|---|
| Protein | BioBrick | Size [kDa] | |
| Firefly luciferase | 61 | ||
| Renilla luciferase | 36 | ||
| NanoLuc luciferase | BBa_K1159001 | 19 | |
| Gaussia luciferase | |||
Transmembrane Domains
Linker domains
| Linker | |||
|---|---|---|---|
| Protein | Sequence | BioBrick | Length [residues] |
| Short Linker | (Gly-Gly-Ser-Gly) | BBa_K243004 | 4 |
| Middle Linker | 2x (Gly-Gly-Ser-Gly) | BBa_K243005 | 8 |
| Long Linker | 3x (Gly-Gly-Ser-Gly) | BBa_K243006 | 12 |
Catalytic domains
Erythromycin Esterase Type II BBa_K1159000
Laccase BBa_K1159002
Laccase TVEL5 BBa_K1615067
TEV Protease BBa_K1159100
Thermonuclease BBa_K1159105
Heroin Esterase BBa_K1615045
CRE recombinase BBa_K1680007
Monoamine Oxidase A BBa_K1615022
Endo-β-Galactosidase BBa_K1483001
α-Galactosidase BBa_K1483002
Acetylgalactosamindase BBa_K1483000
2-oxoglutarate oxygenase/decarboxylase BBa_K1065000
Split proteins
TEV Protease BBa_K1159101 BBa_K1159102
SpyCatcher/SpyTag BBa_K1159200 BBa_K1159201
Binding proteins
Fluorescein binding Anticalin BBa_K1159003
Digoxigenin binding Anticalin BBa_K243003
HER2 binding DARPin BBa_K404302
HER2 binding Affibody BBa_K404302
Nickel Binding Protein (NiBP) BBa_K1321009
Protein tags
Strep-tag II an N-part BBa_K549018
HA tag BBa_K1150016
His6-tag BBa_I757013
SpyTag BBa_K1159020