Difference between revisions of "RFC25 "Freiburg standard" parts"

(→‎Luciferases)
(→‎Reptorter proteins)
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==Linker domains==
 
==Linker domains==
 +
{| class="wikitable"
 +
!colspan="4"|Linker
 +
|-
 +
|Protein
 +
|Sequence
 +
|BioBrick
 +
|Length [residues]
 +
|-
 +
|Short Linker
 +
|(Gly-Gly-Ser-Gly)
 +
|BBa_K243004
 +
|4
 +
|-
 +
|Middle Linker
 +
|2x (Gly-Gly-Ser-Gly)
 +
|BBa_K243005
 +
|8
 +
|-
 +
|Long Linker
 +
|3x (Gly-Gly-Ser-Gly)
 +
|BBa_K243006
 +
|12
 +
|-
 +
|
 +
|
 +
|
 +
|
 +
|-
 +
|
 +
|
 +
|
 +
|
 +
|-
 +
|}
 +
 +
==Catalytic domains==
 +
Erythromycin Esterase Type II
 +
BBa_K1159000
 +
 +
Laccase
 +
BBa_K1159002
 +
 +
TEV Protease
 +
BBa_K1159100
 +
 +
Thermonuclease
 +
BBa_K1159105
 +
 +
==Split proteins==
 +
TEV Protease
 +
BBa_K1159101
 +
BBa_K1159102
 +
 +
SpyCatcher/SpyTag
 +
BBa_K1159200
 +
BBa_K1159201
 +
 +
==Binding proteins==
 +
Fluorescein-Binding Anticalin
 +
BBa_K1159003
 +
</html><groupparts>iGEM013 TU-Munich</groupparts><html>

Revision as of 22:39, 14 May 2016

Reptorter proteins

Fluorescent proteins

Fluorescent proteins
Protein Color BioBrick Excitation [nm] Emission [nm] Quantum Yield
GFP Green BBa_K648013 484 507 0.60
sfGFP Green BBa_K1321337 485 510 0.65
sYFP2 Yellow BBa_K1159301 514 527 0.61
eCFP Cyan BBa_K1159302 439 476 0.40
mRFP Red BBa_K1615089 584 607 0.25

Values for the fluorescent proteins were taken from http://www.microscopyu.com/articles/livecellimaging/fpintro.html.

Luciferases

Luciferases
Protein BioBrick Size [kDa]
Firefly luciferase 61
Renilla luciferase 36
NanoLuc luciferase BBa_K1159001 19
Gaussia luciferase

Transmembrane Domains

Linker domains

Linker
Protein Sequence BioBrick Length [residues]
Short Linker (Gly-Gly-Ser-Gly) BBa_K243004 4
Middle Linker 2x (Gly-Gly-Ser-Gly) BBa_K243005 8
Long Linker 3x (Gly-Gly-Ser-Gly) BBa_K243006 12

Catalytic domains

Erythromycin Esterase Type II BBa_K1159000

Laccase BBa_K1159002

TEV Protease BBa_K1159100

Thermonuclease BBa_K1159105

Split proteins

TEV Protease

BBa_K1159101 BBa_K1159102

SpyCatcher/SpyTag BBa_K1159200 BBa_K1159201

Binding proteins

Fluorescein-Binding Anticalin BBa_K1159003

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