Difference between revisions of "RFC25 "Freiburg standard" parts"
VolkerMorath (Talk | contribs) (→Reptorter proteins) |
VolkerMorath (Talk | contribs) (→Fluorescent proteins) |
||
| Line 13: | Line 13: | ||
|GFP | |GFP | ||
|<span style="background:Lime; color:white;"> Green </span> | |<span style="background:Lime; color:white;"> Green </span> | ||
| − | | | + | |<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_K648013'>BBa_K648013</A></HTML> |
|484 | |484 | ||
|507 | |507 | ||
| Line 20: | Line 20: | ||
|sfGFP | |sfGFP | ||
|<span style="background:Lime; color:white;"> Green </span> | |<span style="background:Lime; color:white;"> Green </span> | ||
| − | | | + | |<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_K1321337'>BBa_K1321337</A></HTML> |
|485 | |485 | ||
|510 | |510 | ||
| Line 27: | Line 27: | ||
|sYFP2 | |sYFP2 | ||
|<span style="background:Yellow; color:black;"> Yellow </span> | |<span style="background:Yellow; color:black;"> Yellow </span> | ||
| − | | | + | |<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_K1159301'>BBa_K1159301</A></HTML> |
|514 | |514 | ||
|527 | |527 | ||
| Line 34: | Line 34: | ||
|eCFP | |eCFP | ||
|<span style="background:DeepPink; color:black;"> Cyan </span> | |<span style="background:DeepPink; color:black;"> Cyan </span> | ||
| − | | | + | |<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_K1159302'>BBa_K1159302</A></HTML> |
|439 | |439 | ||
|476 | |476 | ||
| Line 41: | Line 41: | ||
|mRFP | |mRFP | ||
|<span style="background:red; color:black;"> Red </span> | |<span style="background:red; color:black;"> Red </span> | ||
| − | | | + | |<HTML><A class='part_link' HREF='https://parts.igem.org/wiki/index.php/Part:BBa_K1615089'>BBa_K1615089</A></HTML> |
|584 | |584 | ||
|607 | |607 | ||
|0.25 | |0.25 | ||
|} | |} | ||
| + | |||
Values for the fluorescent proteins were taken from http://www.microscopyu.com/articles/livecellimaging/fpintro.html. | Values for the fluorescent proteins were taken from http://www.microscopyu.com/articles/livecellimaging/fpintro.html. | ||
Revision as of 22:20, 14 May 2016
Contents
Reptorter proteins
Fluorescent proteins
| Fluorescent proteins | |||||
|---|---|---|---|---|---|
| Protein | Color | BioBrick | Excitation [nm] | Emission [nm] | Quantum Yield |
| GFP | Green | BBa_K648013 | 484 | 507 | 0.60 |
| sfGFP | Green | BBa_K1321337 | 485 | 510 | 0.65 |
| sYFP2 | Yellow | BBa_K1159301 | 514 | 527 | 0.61 |
| eCFP | Cyan | BBa_K1159302 | 439 | 476 | 0.40 |
| mRFP | Red | BBa_K1615089 | 584 | 607 | 0.25 |
Values for the fluorescent proteins were taken from http://www.microscopyu.com/articles/livecellimaging/fpintro.html.
Luciferases
| Fluorescent proteins | |||
|---|---|---|---|
| Protein | BioBrick | Size [kDa] | |
| Firefly luciferase | 61 | ||
| Renilla luciferase | 36 | ||
| NanoLuc luciferase | BBa_K1159001 | 19 | |
| Gaussia luciferase | |||