Difference between revisions of "Part:BBa K1321327:Design"
(→‎Design Notes)
 
Line 6: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
BBa_K1321302 was created by restricting BBa_K1033917 (Anderson promoter+RFP insert) and BBa_K1321300 (pSEVA331-BB backbone) with XbaI and PstI, gel purifying the resulting fragments and ligating with T4 ligase. Ligated DNA was then transformed into chemically competent cells, screened via colony PCR and culture PCR, and confirmed by sequencing.
 
−
 
−
 
  
 
===Source===
 
===Source===

Latest revision as of 15:53, 6 May 2016

pTet02


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 4629
    Illegal SpeI site found at 890
    Illegal PstI site found at 1773
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4629
    Illegal SpeI site found at 890
    Illegal PstI site found at 1773
    Illegal NotI site found at 1766
    Illegal NotI site found at 4635
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4629
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 4629
    Illegal SpeI site found at 890
    Illegal PstI site found at 1773
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 4629
    Illegal XbaI site found at 4644
    Illegal SpeI site found at 890
    Illegal PstI site found at 1773
    Illegal NgoMIV site found at 2948
    Illegal AgeI site found at 1469
    Illegal AgeI site found at 1581
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

References